ARTICLE NAME:

STUDIES ON EFFICIENCY OF N2 FIXATION BY Bradyrhizobium japonicum ISOLATED FROM ROOT NODULES OF BLACK GRAM GROWN UNDER PROBLEM SOILS (SALINE)

AUTHOR NAME

N. Sivakumar, K. Krishnapriya, P. Nirmala, A. Chandhru

ISSUE:

VOL.1, NO.1

YEAR:

JUNE 2018

PAGE NO:

34-41

ABSTRACT:

Growth, nodulation and yield response of Black gram [Vigna mungo (L.) Hepper] to inoculation by Bradyrhizobium japonicum. The competitive nodulation ability of a Bradyrhizobium strain is an important property, as strain must compete with other Bradyrhizobia in the rhizosphere for nodulation sites on the host plant. If inoculant strain are to succeed they must have the ability of competitiveness as well as being effective in symbiosis. The establishment of effective nodulation of green gram plant can be enhanced by using effective and competitive strains of Bradyrhizobium. In the present study 30 isolates of Bradyrhizobium japanicum. obtained from saline locations were compared for their N2 fixing efficiency based on IAA production, EPS production, nodulation, nodule ARA activity and nodule N content. The isolate obtained from vallampadugai (GPJ-18) produced maximum of IAA, EPS and ARA activity and this recorded the highest number of 25.00 nodules plant-1 ARA activity of 202.50 moles C2H4 formed h-1 g -1 and nodule nitrogen content of 5.75%.

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